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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
21/02/2014 |
Actualizado : |
26/02/2018 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
CASTRO, A.; GERMAN, S.; GONZÁLEZ, S.N.; HAYES, P.M.; PEREYRA, S.; PEREZ, C. |
Afiliación : |
EEMAC, Facultad de Agronomía, UdelaR, Paysandú, Uruguay.; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA NOEMI GONZALEZ PARODI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Barley project, Department of Crop and Soil Sciences, Oregon State University, Corvallis OR 97331, USA.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EEMAC, Facultad de Agronomía, UdelaR, Paysandú, Uruguay. |
Título : |
QTL analysis of spot blotch and leaf rust resistance in the BCD47 × Baronesse barley mapping population. |
Fecha de publicación : |
2008 |
Fuente / Imprenta : |
International Barley Genetics Symposium,10.,Alexandria, Egypt: ICARDA, Proceedings, 2008. |
Páginas : |
p. 311-319. |
Idioma : |
Inglés |
Contenido : |
Abstract
Leaf rust (LR) (caused by Puccinia hordei) and spot blotch (SB) (caused by Cochliobolus
sativus) are two of the main diseases of barley in Uruguay. We studied the genetics
of the resistance to both diseases present in a doubled-haploid (DH) population derived
from the cross BCD47 × Baronesse. BCD47 has low SB severity and high susceptibility to
LR, while Baronesse is susceptible to SB and has low susceptibility to LR. Both resistances
were expressed at the adult plant stage. The population was phenotyped in 9 environments
for each disease. Four QTLs were detected for SB, on chromosomes 1H, 3H, 6H and
7H. In three of them, BCD47 contributed the resistant alleles. The QTLs on chromosome
1H (located in the Bmac213-Bmag770 interval) and chromosome 3H (located in the
Bmag225-Bmag013 interval) were the most consistent across environments. Two main
QTLs were detected for LR on chromosomes 6H (Baronesse contributing the resistant
allele) and 7H (BCD47 contributing the resistant allele), coincident with SB
resistance QTLs. The QTL on chromosome 6H (linked to the SSR Bmag173) was the
most consistent across environments. |
Palabras claves : |
RESISTENCIA A LA ROYA DE LA HOJA. |
Thesagro : |
CEBADA; ROYA. |
Asunto categoría : |
H20 Enfermedades de las plantas |
Marc : |
LEADER 01814naa a2200229 a 4500 001 1041375 005 2018-02-26 008 2008 bl uuuu u00u1 u #d 100 1 $aCASTRO, A. 245 $aQTL analysis of spot blotch and leaf rust resistance in the BCD47 × Baronesse barley mapping population. 260 $c2008 300 $ap. 311-319. 520 $aAbstract Leaf rust (LR) (caused by Puccinia hordei) and spot blotch (SB) (caused by Cochliobolus sativus) are two of the main diseases of barley in Uruguay. We studied the genetics of the resistance to both diseases present in a doubled-haploid (DH) population derived from the cross BCD47 × Baronesse. BCD47 has low SB severity and high susceptibility to LR, while Baronesse is susceptible to SB and has low susceptibility to LR. Both resistances were expressed at the adult plant stage. The population was phenotyped in 9 environments for each disease. Four QTLs were detected for SB, on chromosomes 1H, 3H, 6H and 7H. In three of them, BCD47 contributed the resistant alleles. The QTLs on chromosome 1H (located in the Bmac213-Bmag770 interval) and chromosome 3H (located in the Bmag225-Bmag013 interval) were the most consistent across environments. Two main QTLs were detected for LR on chromosomes 6H (Baronesse contributing the resistant allele) and 7H (BCD47 contributing the resistant allele), coincident with SB resistance QTLs. The QTL on chromosome 6H (linked to the SSR Bmag173) was the most consistent across environments. 650 $aCEBADA 650 $aROYA 653 $aRESISTENCIA A LA ROYA DE LA HOJA 700 1 $aGERMAN, S. 700 1 $aGONZÁLEZ, S.N. 700 1 $aHAYES, P.M. 700 1 $aPEREYRA, S. 700 1 $aPEREZ, C. 773 $tInternational Barley Genetics Symposium,10.,Alexandria, Egypt: ICARDA, Proceedings, 2008.
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
20/07/2022 |
Actualizado : |
20/07/2022 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. |
Afiliación : |
MARTINA CRISPO, Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.; VANESSA CHENOUARD, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; PEDRO DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; LAURENT TESSON, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; JEAN-MARIE HESLAN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; IGNACIO ANEGÓN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
Título : |
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. |
Complemento del título : |
Chapter 12. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12 |
DOI : |
10.1007/978-1-0716-2301-5_12 |
Idioma : |
Inglés |
Contenido : |
Abstract:
CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. |
Palabras claves : |
Embryo; Genome editing; In vitro fertilization; OTOF; SsODNs. |
Thesagro : |
OVEJA. |
Asunto categoría : |
-- |
Marc : |
LEADER 01915naa a2200301 a 4500 001 1063442 005 2022-07-20 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1007/978-1-0716-2301-5_12$2DOI 100 1 $aCRISPO, M. 245 $aGeneration of a Human Deafness Sheep Model Using the CRISPR/Cas System.$h[electronic resource] 260 $c2022 520 $aAbstract: CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. 650 $aOVEJA 653 $aEmbryo 653 $aGenome editing 653 $aIn vitro fertilization 653 $aOTOF 653 $aSsODNs 700 1 $aCHENOUARD, V. 700 1 $aDOS SANTOS-NETO P 700 1 $aTESSON, L. 700 1 $aSOUZA-NEVES, M. 700 1 $aHESLAN, J.M. 700 1 $aCUADRO, F. 700 1 $aANEGÓN, I. 700 1 $aMENCHACA, A. 773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12
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